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Each freeze-thaw cycle creates ice crystals that can physically disrupt peptide structure, and the process concentrates the peptide at the boundary between frozen and unfrozen regions, potentially causing aggregation

bFGF, which is one of the 22 different isotypes of FGF, plays a crucial role in the wound healing process by promoting fibroblast proliferation, inducing neovascularization, and increasing the synthesis of collagenase.3033 As the topical administration of human recombinant bFGF has been shown to be effective for wound healing in clinical situations, we selected bFGF as a positive reagent in this study

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